HSP70 Mouse mAb-科鹿（武漢）生物科技有限責任公司

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產品中心

HSP70 Mouse mAb

EM1042

規格：	價格：
50 μl	￥1280.00
100 μl	￥1980.00

下載說明 ①

Overview

Product name:	HSP70 Mouse mAb
Alternative Names:	Heat shock 70 kDa protein 1/2, heat shock 70kDa protein 1A, HSP70, HSP70 1, HSP70 1/HSP70 2, HSP70 1A, HSP70.1/HSP70.2, HSP70I, HSP72, HSPA1, HSPA1A, HSPA1B
Source:	Mouse
Applications:	WB, IHC, IF
Reactivity:	Human, Rat, Mouse
Dilutions:	WB 1:1,000-2,000 IF 1:100-200? IHC 1:200-500
Immunogen:	Synthetic Peptide
Storage:	PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid?repeated?freeze-thaw?cycles.
Clonality:	Monoclonal
Isotype:	IgG1
Concentration:	1mg/ml
Observed Band:	70kDa
GeneID（Human）:	3303
Human Swiss-Prot No:	P0DMV8
Cellular localization:	Cytoplasm
Background:	The 70 kilodalton heat shock proteins (Hsp70s) are a family of ubiquitously expressed heat shock proteins. Proteins with similar structure exist in virtually all living organisms. The Hsp70s are an important part of the cell's machinery for protein folding, and help to protect cells from stress. Hsp70 is usually in an ATP bound state. Hsp70 by itself is characterized by a very weak ATPase activity, such that spontaneous hydrolysis will not occur for many minutes. As newly synthesized proteins emerge from the ribosomes, the substrate binding domain of Hsp70 recognizes sequences of hydrophobic amino acid residues, and interacts with them. This spontaneous interaction is reversible, and in the ATP bound state Hsp70 may relatively freely bind and release peptides. However, the presence of a peptide in the binding domain stimulates the ATPase activity of Hsp70, increasing its normally slow rate of ATP hydrolysis.

Western blot analysis of 1) Hela, 2) Mouse Brain, with HSP70 mAb diluted at 1:2,000.
Western blot analysis of?extracts from HeLa (Lane 1),MCF-7 (Lane 2), using HSP70 diluted?at?1:2,000.
IF analysis of Hela with EM1042(Left) and DAPI (Right) diluted at 1:100.
Immunohistochemical analysis of paraffin-embedded Human Lung caricnoma using HSP70 （EM1042）Mouse mAb diluted at 1:500.

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