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人角蛋白10(KRT10)酶聯免疫吸附檢測試劑盒
ELK2149
規格: 價格:
48 T ¥1960.00
96 T ¥2800.00

Overview

Product name: Human KRT10(Keratin 10) ELISA Kit
Reactivity: Human
Alternative Names: K10; CK10; KPP; Cytokeratin 10; Epidermolytic Hyperkeratosis; Keratosis Palmaris Et Plantaris; Keratin, type I cytoskeletal 10
Assay Type: Sandwich
Sensitivity: 6.12 ng/mL
Standard: 1000 ng/mL
Detection Range: 15.63-1000 ng/mL
Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5h
Research Area: Tumor immunity;Infection immunity;Dermatology;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human KRT10. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human KRT10. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human KRT10, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human KRT10 in the samples is then determined by comparing the OD of the samples to the standard curve.

標準曲線

Concentration (ng/mL) OD Corrected OD
1000.00 2.018 1.929
500.00 1.594 1.505
250.00 1.196 1.107
125.00 0.682 0.593
62.50 0.487 0.398
31.25 0.385 0.296
15.63 0.168 0.079
0.00 0.089 0.000

精密度

Intra-assay Precision (Precision within an assay):CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays):CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant KRT10 and the recovery rates were calculated by comparing the measured value to the expected amount of KRT10 in samples.
Matrix Recovery range Average
serum(n=5) 89-105% 97%
EDTA plasma(n=5) 78-90% 84%
Heparin plasma(n=5) 84-97% 91%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of KRT10 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 95-104% 82-96% 92-105% 83-92%
EDTA plasma(n=5) 83-92% 85-96% 93-99% 95-102%
Heparin plasma(n=5) 80-97% 81-92% 87-103% 82-93%
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