<td id="jxiji"></td>
<strike id="jxiji"><dfn id="jxiji"><strike id="jxiji"></strike></dfn></strike>

<strike id="jxiji"><dfn id="jxiji"></dfn></strike>

<button id="jxiji"><xmp id="jxiji"><strike id="jxiji"></strike>

<button id="jxiji"></button>

<strike id="jxiji"><dfn id="jxiji"></dfn></strike>

<strike id="jxiji"><dfn id="jxiji"></dfn></strike>
EN

產品中心

小鼠脂蛋白(Lp-a)酶聯免疫吸附檢測試劑盒
ELK0410
規格: 價格:
48 T ¥2240.00
96 T ¥3200.00

Overview

Product name: Mouse Lp-a(Lipoprotein a) ELISA Kit
Reactivity: Mouse
Alternative Names: LP; AK38; APOA
Assay Type: Sandwich
Sensitivity: 0.61 ng/mL
Standard: 100 ng/mL
Detection Range: 1.57-100 ng/mL
Sample Type: Serum, plasma and other biological fluids.
Assay Length: 3.5h
Research Area: Metabolic pathway;Cardiovascular biology;Hepatology;Gastroenterology;Nutrition metabolism;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse Lp-a. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse Lp-a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse Lp-a, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse Lp-a in the samples is then determined by comparing the OD of the samples to the standard curve.

標準曲線

Concentration (ng/mL) OD Corrected OD
100.00 2.132 2.037
50.00 1.628 1.533
25.00 1.096 1.001
12.50 0.963 0.868
6.25 0.595 0.500
3.13 0.331 0.236
1.57 0.235 0.140
0.00 0.095 0.000

精密度

Intra-assay Precision (Precision within an assay):CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays):CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant Lp-a and the recovery rates were calculated by comparing the measured value to the expected amount of Lp-a in samples.
Matrix Recovery range Average
serum(n=5) 95-107% 101%
EDTA plasma(n=5) 87-99% 93%
Heparin plasma(n=5) 82-95% 88%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Lp-a and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 97-105% 88-95% 96-102% 83-97%
EDTA plasma(n=5) 95-103% 88-96% 81-95% 91-102%
Heparin plasma(n=5) 89-97% 86-102% 94-106% 85-97%
關閉

在線咨詢

Online consultation

  • 在線咨詢
  • 技術支持

關注微信公眾號

微信掃一掃立即咨詢

微信掃一掃立即咨詢

又粗又色又爽一区二区三区,成人精品视频,av天堂首页,你懂得在线
<td id="jxiji"></td>
<strike id="jxiji"><dfn id="jxiji"><strike id="jxiji"></strike></dfn></strike>

<strike id="jxiji"><dfn id="jxiji"></dfn></strike>

<button id="jxiji"><xmp id="jxiji"><strike id="jxiji"></strike>

<button id="jxiji"></button>

<strike id="jxiji"><dfn id="jxiji"></dfn></strike>

<strike id="jxiji"><dfn id="jxiji"></dfn></strike>