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人碘甲腺原氨酸脫碘酶Ⅲ(DIO3)酶聯免疫吸附檢測試劑盒
ELK0408
規格: 價格:
48 T ¥2240.00
96 T ¥3200.00

Overview

Product name: Human DIO3(Iodothyronine Deiodinase III) ELISA Kit
Reactivity: Human
Alternative Names: ITDI3; TXDI3; Type-III 5'-deiodinase
Assay Type: Sandwich
Sensitivity: 0.11 ng/mL
Standard: 20 ng/mL
Detection Range: 0.32-20 ng/mL
Sample Type: Tissue homogenates and other biological fluids.
Assay Length: 3.5h
Research Area: Enzyme & Kinase;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human DIO3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human DIO3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human DIO3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human DIO3 in the samples is then determined by comparing the OD of the samples to the standard curve.

標準曲線

Concentration (ng/mL) OD Corrected OD
20.00 2.368 2.273
10.00 1.563 1.468
5.00 1.012 0.917
2.50 0.755 0.660
1.25 0.515 0.420
0.63 0.397 0.302
0.32 0.161 0.066
0.00 0.095 0.000

精密度

Intra-assay Precision (Precision within an assay):CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays):CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant DIO3 and the recovery rates were calculated by comparing the measured value to the expected amount of DIO3 in samples.
Matrix Recovery range Average
serum(n=5) 82-95% 88%
EDTA plasma(n=5) 89-103% 96%
Heparin plasma(n=5) 78-93% 85%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of DIO3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 81-93% 86-98% 91-103% 82-95%
EDTA plasma(n=5) 87-96% 91-101% 87-98% 89-103%
Heparin plasma(n=5) 84-94% 85-103% 94-105% 92-101%
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